a sma positive cells  (Oxford Instruments)

Journal: Scientific Reports

Article Title: The telomerase activator TA-65 protects from cigarette smoke-induced small airway remodeling in mice through extra-telomeric effects

doi: 10.1038/s41598-022-25993-7

Figure Lengend Snippet: TA-65 effects on cigarette smoke-induced small airway remodeling. Representative lung tissue sections of mice exposed to air or cigarette-smoke (CS) with or without TA-65, and stained for HE, picrosirius red, α-SMA, PAS, Collagen I, Collagen III, or Vimentin ( a ). Quantification of small airways wall thickness by manual [( b ), in mean] or automated method [( c ), distribution], α-SMA expression ( d ), PAS staining ( e ), Collagen I ( f ), Collagen III ( g ) and Vimentin ( h ) expression. Original magnification, ×20. n = 7–8 mice per group. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Each culture contained between 8 to 10% of α-SMA positive cells (1:200, Sigma A5228).

Techniques: Staining, Expressing

Journal: Scientific Reports

Article Title: The telomerase activator TA-65 protects from cigarette smoke-induced small airway remodeling in mice through extra-telomeric effects

doi: 10.1038/s41598-022-25993-7

Figure Lengend Snippet: Imetelstat effects on cigarette smoke-induced small airway remodeling. Representative lung tissue sections of mice exposed to air or cigarette-smoke (CS) with or without Imetelstat, and stained for HE, picrosirius red, α-SMA, or PAS ( a ). Quantification of small airways wall thickness [mean in ( b ), distribution in ( c )], α-SMA expression ( d ), PAS staining ( e ). Original magnification, ×20. n = 7–8 mice per group. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01.

Article Snippet: Each culture contained between 8 to 10% of α-SMA positive cells (1:200, Sigma A5228).

Techniques: Staining, Expressing

Journal: Scientific Reports

Article Title: The telomerase activator TA-65 protects from cigarette smoke-induced small airway remodeling in mice through extra-telomeric effects

doi: 10.1038/s41598-022-25993-7

Figure Lengend Snippet: Effects of TA-65 on TGF-β-induced fibroblasts-to-myofibobroblasts differentiation in vitro. Mouse primary lung fibroblasts (MPLF) and MEF cells were pre-treated with TA-65 (2 µM) or Imetelstat (1 µM) during 24 h, followed by TGF-β treatment (10 ng/mL) for 48 h. Histograms show quantification of α-SMA expression evaluated by immunostaining ( a : MPLF; b : MEF). Representative images of cells stained with α-SMA monoclonal antibody are available in Supplementary Fig. . n = 3 samples per group. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01.

Article Snippet: Each culture contained between 8 to 10% of α-SMA positive cells (1:200, Sigma A5228).

Techniques: In Vitro, Expressing, Immunostaining, Staining

Journal: International Journal of Neonatal Screening

Article Title: Development of a Multiplex Real-Time PCR Assay for the Newborn Screening of SCID, SMA, and XLA

doi: 10.3390/ijns5040039

Figure Lengend Snippet: Amplification plots for: ( a ) A reference SMA positive sample with three copies of the SMN2 gene showing no amplification of the SMN1 locus; ( b ) an individual over 55 years old (SCID-like) showing no amplification for the TREC locus and normal amplification for the other three targets; ( c ) a contrived SMA, SCID, and XLA positive sample showing amplification only for RPP30. ( d ) A reference SMA carrier sample showing robust amplification of the SMN1 locus.

Article Snippet: Contrived SMA, SCID, and XLA positive samples were made by spiking washed leukocyte depleted blood with SMA positive cells obtained from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research (Repository ID GM23689).

Techniques: Amplification